Elisa Microwell Blocking Buffer With Stabilizer (Azide And Mercury Free)

• Catalog Number: orb348584
• Category: Assays and Kits
• Description: Elisa Microwell Blocking Buffer With Stabilizer (Azide And Mercury Free)
• Tested applications: ELISA
• Concentration: 1X
• Dilution range: ELISA: User Optimized
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
• Conjugation: Unconjugated
• Hazard Information: Non-Toxic
• Storage: Store container at 4° C before opening. Protect from moisture and light. No special shipping conditions or precautions are required.
• Buffer/Preservatives: See application note.
• Alternative names: ELISA Microwell Blocking Buffer with Stabilizer (A
• Note: For research use only
• Application notes: This product is a ''ready-to-use'' 1X solution for stabilizing ELISA plates for storage prior to ELISA. After coating with antigen or antibody, wash the contents of the ELISA microwells and add a sufficient volume of ELISA Microwell Blocking Buffer with Stabilizer to each well of the microplate. Let stand for 2h at room temperature. Aspirate contents of each well and allow the plate to dry. Seal the plate and store appropriately for future use. This buffer contains Tris buffered saline and proprietary reagents to block and stabilize ELISA plates. A proprietary combination of stabilizers and preservatives are used that is azide and mercury free.
• Expiration Date: Please enquire.

ELISA results of ELISA Microwell Blocking Buffer with Stabilizer (Azide and Mercury Free). Each well was coated in duplicate with 1.0 µg of Mouse IgG Whole Molecule. Blocking Buffer (Red), Blocking Buffer reference (Green), and Blocking Buffer control (Blue). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Rabbit Anti-Mouse IgG, Goat anti-Rabbit HRP 1:8500, and TMB substrate.