Elisa Coating Stabilizer

• Catalog Number: orb348582
• Category: Assays and Kits
• Description: Elisa Coating Stabilizer
• Tested applications: ELISA
• Concentration: 1X
• Dilution range: ELISA: User Optimized
• Form/Appearance: Liquid (sterile filtered)
• Purity: ELISA Plate Coating Stabilizer was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
• Conjugation: Unconjugated
• Hazard Information: Non-Toxic
• Storage: Store container at 4° C before opening. Protect from moisture and light. No special shipping conditions or precautions are required.
• Buffer/Preservatives: See application note.
• Alternative names: ELISA Microwell Coating Stabilizer (Azide and Merc
• Note: For research use only
• Application notes: ELISA Microwell Coating Stabilizer is a ''ready-to-use'' 1X solution for stabilizing ELISA plates for storage prior to preforming ELISA. After the addition of blocking agent, wash the contents of ELISA microwells and add a sufficient volume of ELISA Microwell Coating Stabilizer to each well of the microplate. Let stand for 30 minutes at room temperature. Aspirate contents of each well and allow the plate to dry. Store appropriately for future use. This buffer contains phosphate buffered saline and proprietary reagents to stabilize and preserve ELISA plates. This buffer is a proprietary combination of stabilizers and preservatives that is azide and mercury free.
• Expiration Date: Please enquire.

Biorbyt produces a wide variety of buffers and substrates for use in ELISAs. Antigen was diluted in ELISA Microwell Coating Stabilizer (p/n orb348581) added to the microwell plate and incubated overnight at 4°C. The plate was then blocked with ELISA Microwell Blocking Buffer with Stabilizer (p/n orb348584) for 2 hours. The primary antibody was diluted in PBS Fish Gel Concentrate (1:10)(p/n orb348587), added to the plate, and allowed to incubate 1 hour at room temperature. HRP conjugated secondary antibody was diluted in HRP Conjugate Stabilizer, added to the plate, and allowed to incubate for 30 minutes at room temperature. TMB ELISA Peroxidase Substrate (p/n orb348651) was added to the plate and allowed to incubate for 30 minutes at room temperature. The reaction was then stopped with 1M HCl and read at 450nm.