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Catalog Number | orb1274490 |
---|---|
Category | Antibodies |
Description | ELAVL1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human HuR / ELAVL1 (NP_001410.2). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 37kDa |
Target | ELAVL1 |
UniProt ID | Q15717 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | ELAVL1, ELAV1, Hua, HUR, Hu antigen R, ELAV-like p Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using HuR/ELAVL1 antibody (orb1274490) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and HuR/ELAVL1 knockout (KO) 293T cells, using HuR/ELAVL1 antibody (orb1274490) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse testis using HuR/ELAVL1 antibody (orb1274490) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using HuR/ELAVL1 antibody (orb1274490) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using HuR/ELAVL1 antibody (orb1274490) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using HuR/ELAVL1 antibody (orb1274490) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using HuR/ELAVL1 antibody (orb1274490) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of MCF-7 cells using HuR/ELAVL1 antibody (orb1274490). Blue: DAPI for nuclear staining.
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC, WB | |
Human, Mouse | |
Polyclonal | |
Unconjugated |
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