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Catalog Number | orb867270 |
---|---|
Category | Antibodies |
Description | Guinea pig polyclonal antibody to Elastin, human |
Species/Host | Guinea pig |
Tested applications | ELISA, IF, IHC-P, RIA |
Immunogen | Purified elastin from human skin |
Concentration | app. 1 mg/ml |
Dilution range | (purified, lyophilized) IFA ? 1:80, IHC(P) ? 1:500 - 1:1000, ELISA ? 1:200 (OD ? 500) |
Conjugation | Unconjugated |
Hazard Information | *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. |
UniProt ID | P15502 |
Storage | Storage: -20°C |
Buffer/Preservatives | Affinity purified antibody lyophilized from phosphate buffered solution; no rAlbumin and preservative added! |
Note | For research use only |
Application notes | Positive Control: Human skin or aorta. Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Incubation Time: IHC(P) 60 min at RT or 2-8°C over night |
Expiration Date | 12 months from date of receipt. |
IHC-P | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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