EIF6 Antibody (monoclonal, 2I11)

• Catalog Number: orb527047
• Category: Antibodies
• Description: EIF6 Antibody (monoclonal, 2I11)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2I11
• Tested applications: FC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG2a
• Immunogen: E.coli-derived human EIF6 recombinant protein (Position: N66-T210). Human EIF6 shares 99.3% amino acid (aa) sequence identity with both mouse and rat EIF6.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 26 kDa
• UniProt ID: P56537
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Eukaryotic translation initiation factor 6; eIF-6;
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U20S cells using anti-EIF6 antibody (Blue line).Isotype control antibody (Green line) was mouse IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis using anti-EIF6 antibody.Lane 1:human placenta tissue; 2:CACO-2 cell; 3:HEPG2 cell; 4:K562 cell; 5:human A549 cell; 6:human PC-3 cell; 7:human HeLa cell; 8:human U87 cell; 9:rat kidney tissue; 10:rat testis tissue; 11:mouse HEPA1-6 cell.

IHC analysis of EIF6 using anti-EIF6 antibody. EIF6 was detected in paraffin-embedded section of human intestinal cancer tissue.

IHC analysis of EIF6 using anti-EIF6 antibody. EIF6 was detected in paraffin-embedded section of human mammary cancer tissue.

IHC analysis of EIF6 using anti-EIF6 antibody. EIF6 was detected in paraffin-embedded section of human lung cancer tissue.

IHC analysis of EIF6 using anti-EIF6 antibody. EIF6 was detected in paraffin-embedded section of rat spleen tissue.