eIF5B/IF2 Antibody

• Catalog Number: orb1527866
• Category: Antibodies
• Description: eIF5B/IF2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human
• Immunogen: between 450 and 500
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - Not recommended. Use rabbit anti-eIF5B/IF2 antibody A301-744A.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: eIF5B/IF2
• UniProt ID: O60841
• NCBI: NP_056988.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: eukaryotic translation initiation factor 5B; IF2;
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human eIF5B/IF2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-eIF5B/IF2 antibody orb1527866 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. eIF5B/IF2 was immunoprecipitated successfully when using rabbit anti-eIF5B/IF2 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).