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    EIF4G2 antibody

    Catalog Number: orb213886

    DispatchUsually dispatched within 5-10 working days
    $ 337.00
    Catalog Numberorb213886
    CategoryAntibodies
    DescriptionRabbit polyclonal antibody to EIF4G2
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsIF, IH, WB
    ReactivityHuman, Mouse, Primate, Rabbit, Rat
    ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human EIF4G2. The exact sequence is proprietary.
    Dilution rangeWB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500
    Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    ConjugationUnconjugated
    TargetEIF4G2
    Entrez13690, 1982
    UniProt IDP78344, Q62448
    SourceRabbit
    StorageShipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
    Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    Alternative namesanti DAP5 antibody, anti Eukaryotic translation in
    Read more...
    NoteFor research use only
    Expiration Date12 months from date of receipt.
    EIF4G2 antibody

    Western blot analysis of EIF4G2 expression in Hela (A), U2OS (B), mouse liver (C) whole cell lysates. (Predicted band size: 102 kD; Observed band size: 102 kD)

    EIF4G2 antibody

    Immunohistochemical analysis of EIF4G2 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    EIF4G2 antibody

    Immunofluorescent analysis of EIF4G2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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