eIF4E (phospho Ser209) rabbit pAb

• Catalog Number: orb764180
• Category: Antibodies
• Description: Rabbit polyclonal antibody to eIF4E (phospho-Ser209)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Immunogen: The antiserum was produced against synthesized peptide derived from human eIF4E around the phosphorylation site of Ser209. AA range:168-217
• MW: 25
• Tested applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-EIF4E antibody, anti-EIF4EL1 antibody, anti-E
• Note: For research use only

Western Blot analysis of various cells using Phospho-eIF4E (S209) Polyclonal Antibody.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using eIF4E (Phospho-Ser209) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from 293 cells treated with Anisomycin 25ug/ml 30', using eIF4E (Phospho-Ser209) Antibody. The lane on the right is blocked with the phospho peptide.