eIF4AIII/EIF4A3 Antibody

• Catalog Number: orb1525659
• Category: Antibodies
• Description: eIF4AIII/EIF4A3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 125 and 175
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: eIF4AIII/EIF4A3
• UniProt ID: P38919
• NCBI: NP_055555.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: NMP 265; RCPS; NUK34; NMP265; MUK34; hNMP 265; Fal
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse eIF4AIII/EIF4A3 by western blot. Samples: Whole cell lysate from HeLa (5, 15, and 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibody: Affinity purified rabbit anti-eIF4AIII/EIF4A3 antibody orb1525659 used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human eIF4AIII/EIF4A3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-eIF4AIII/EIF4A3 antibody orb1525659 used for IP at 3 µg/mg lysate.

Detection of human eIF4AIII/EIF4A3 by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-eIF4AIII/EIF4A3 antibodies. Detection: DAB

Detection of mouse eIF4AIII/EIF4A3 by immunohistochemistry.Sample: FFPE section of mouse renal carcinoma. Antibody: Affinity purified rabbit anti-eIF4AIII/EIF4A3 (Cat. No. orb1525659) used at a dilution of 1: 200 (1µg/ml). Detection: DAB