EIF4A1 Antibody

• Catalog Number: orb623798
• Category: Antibodies
• Description: EIF4A1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence of human EIF4A1 (LQQIELDLKATQALVLA).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 46 kDa
• UniProt ID: P60842
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Eukaryotic initiation factor 4A-I; eIF-4A-I; eIF4A
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HepG2 cells using anti-EIF4A1 antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG.Unlabelled sample (Red line) was also used as a control.

IF analysis of EIF4A1 using anti-EIF4A1 antibody.

IHC analysis of EIF4A1 using anti-EIF4A1 antibody.

IHC analysis of EIF4A1 using anti-EIF4A1 antibody.

IHC analysis of EIF4A1 using anti-EIF4A1 antibody.

WB analysis using anti-EIF4A1 antibody.Lane 1:placenta tissue, Lane 2:HEK293 cell, Lane 3:A549 cell, Lane 4:HepG2 cell, Lane 5:U-87MG cell, Lane 6:rat lung tissue, Lane 7:mouse spleen tissue.