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    eIF3J/EIF3S1 Antibody

    Catalog Number: orb1527864

    DispatchUsually dispatched within 5-10 working days
    $ 220.00
    Catalog Numberorb1527864
    CategoryAntibodies
    DescriptioneIF3J/EIF3S1 Antibody
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsIHC, IP, WB
    ReactivityHuman, Mouse
    Immunogenbetween 208 and 258
    Concentration1000 µg/ml
    Dilution rangeWB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
    Form/AppearanceWhole IgG
    ConjugationUnconjugated
    TargeteIF3J/EIF3S1
    UniProt IDO75822
    NCBINP_003749.2
    Storage2 - 8°C
    Buffer/PreservativesTris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
    Alternative nameseIF3j; eukaryotic translation initiation factor 3,
    Read more...
    NoteFor research use only
    Application notesFormat: Whole IgG
    Expiration Date12 months from date of receipt.
    eIF3J/EIF3S1 Antibody

    Detection of human eIF3J/EIF3S1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-eIF3J/EIF3S1 antibody orb1527864 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. eIF3J/EIF3S1 was also immunoprecipitated by rabbit anti-eIF3J/EIF3S1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

    eIF3J/EIF3S1 Antibody

    Detection of human and mouse eIF3J/EIF3S1 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-eIF3J/EIF3S1 (Cat. No. orb1527864) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

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      Polyclonal

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      200 μl, 100 μl, 50 μl
    • eIF3J/EIF3S1 Antibody [orb1527865]

      IHC,  IP,  WB

      Human, Mouse

      Rabbit

      Polyclonal

      Unconjugated

      100 μg
    • eIF3α antibody [orb1413873]

      WB

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      Rabbit

      Polyclonal

      Unconjugated

      100 μl
    • eIF3J/EIF3S1 Antibody [orb1517769]

      IHC

      Human, Mouse

      Rabbit

      Polyclonal

      Unconjugated

      1 μg
    • eIF3J/EIF3S1 Antibody [orb1517770]

      IHC

      Human, Mouse

      Rabbit

      Polyclonal

      Unconjugated

      10 μg
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