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Catalog Number | orb411705 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to EIF3B |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Rat |
Immunogen | Recombinant full length protein of human EIF3B |
Dilution range | WB: 1:500-2000 |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | EIF3B |
Entrez | 27979, 8662, 288516 |
UniProt ID | P55884, Q8JZQ9, Q4G061 |
Source | Rabbit |
Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | anti-EIF3S9 antibody, anti-Eukaryotic translation Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of EIF3B expression in Hela (A), Jurkat (B), NIH3T3 (C), rat thymus (D) whole cell lysates. (Predicted band size: 92; 99 kD; Observed band size: 125 kD)
Immunofluorescent analysis of EIF3B staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
ICC, IP, WB | |
Human, Monkey, Mouse | |
Rabbit | |
Monoclonal | |
Unconjugated |
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