eIF2α/EIF2S1 Antibody

• Catalog Number: orb1294329
• Category: Antibodies
• Description: Rabbit polyclonal antibody to eIF2 alpha
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Rabbit
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the center region
• Dilution range: Western Blot 1:500 Immunofluorescence 1:200–1:500 Immunohistochemistry (Paraffin) 1:100-1:150
• Conjugation: Unconjugated
• Target: eIF2α / EIF2S1
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 1%BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-eIF2 alpha antibody - at 1/500 dilution, Lysates at 50 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA an hour before being incubated with orb1294329 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294329 Anti-eIF2 alpha antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded breast tissue labeling eIF2 alpha with orb1294329 at 1/100.