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Catalog Number | orb1526416 |
---|---|
Category | Antibodies |
Description | eIF2B5 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP |
Reactivity | Human |
Immunogen | between 550 and 600 |
Concentration | 1000 µg/ml |
Dilution range | WB - Not recommended. Use rabbit anti-eIF2B5 antibody A302-556A.; IP - 5 - 15 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | eIF2B5 |
UniProt ID | Q13144 |
NCBI | NP_003898.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | CACH; LVWM; eukaryotic translation initiation fact Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human eIF2B5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-eIF2B5 antibody orb1526416 used for IP at 10 µg/mg lysate.
Detection of human eIF2B5 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-eIF2B5 (Cat. No. orb1526416) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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