EIF2B3 Mouse Monoclonal Antibody

• Catalog Number: orb783004
• Category: Antibodies
• Description: EIF2B3 Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: H11F4
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1,k
• Immunogen: KLH conjugated synthetic peptide derived from human EIF2B3 (151-400/452aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:25, ICC/IF=1:25, IF=1:25
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 50 kDa
• Target: EIF2B3
• UniProt ID: Q9NR50
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: EI2BG_HUMAN; EIF 2B; EI2BG; eIF 2B GDP GTP exchang
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: HL-60 cell lysates, Lane 2: MCF-7 cell lysates, Lane 3: U-2OS cell lysates, Lane 4: K562 cell lysates, Primary: Anti-EIF2B3 (orb783004) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 56 kD.

U-2 OS cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (EI2BG) monoclonal Antibody, Unconjugated (orb783004) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. Dylight® 554 Phalloidin (red) was used to stain the cell Cytoplasmic actin.