eIF2B delta/EIF2B4 Antibody

• Catalog Number: orb1525656
• Category: Antibodies
• Description: eIF2B delta/EIF2B4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: between 50 and 100
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: eIF2B delta/EIF2B4
• UniProt ID: Q9UI10
• NCBI: NP_001029288.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: EIF2B; translation initiation factor eIF-2B subuni
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human eIF2B delta/EIF2B4 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-eIF2B delta/EIF2B4 antibody orb1525656 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 6 µg/mg lysate. eIF2B delta/EIF2B4 was also immunoprecipitated by rabbit anti-eIF2B delta/EIF2B4 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Detection of human eIF2B delta/EIF2B4 by immunohistochemistry. Samples: FFPE sections of human colon carcinoma and breast carcinoma. Antibody: Affinity purified rabbit anti-eIF2B delta/EIF2B4 (Cat. No. orb1525656) used at a dilution of 1: 200 (1µg/ml). Detection: DAB