EIF2A antibody

• Catalog Number: orb153846
• Category: Antibodies
• Description: Rabbit polyclonal antibody to EIF2A. Phosphorylation of the eukaryotic initiation factor 2 (eIF2) alpha subunit is a welldocumented mechanism to downregulate protein synthesis under a variety of stress conditions. eIF2 binds GTP and MettRNAi and transfers MettRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the alpha subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2GDPeIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN and TNF alpha induces potent phosphorylation of eIF2 alpha at Ser51
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein of human eIF2α(NP_004085.1).
• Dilution range: WB: 1:500-1000, IHC-P: 1:50-100, IF/ICC: 1:20-50, IP: 1:20-50, FACS: 1:20-50
• Conjugation: Unconjugated
• MW: 38KDa
• UniProt ID: P05198
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Alternative names: anti EIF2S1 antibody, anti EIF2 antibody, anti EIF
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cells using EIF2A antibody