EHMT1 Antibody

• Catalog Number: orb1528041
• Category: Antibodies
• Description: EHMT1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: between 25 and 75
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: EHMT1
• UniProt ID: Q9H9B1
• NCBI: NP_079033.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: G9a-like protein 1; KLEFS1; histone-lysine N-methy
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human EHMT1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, A-549, K-562, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EHMT1 antibody (orb1528041) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human EHMT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EHMT1 antibody (orb1528041) used for IP at 6 µg per reaction. EHMT1 was also immunoprecipitated by a previous lot of this antibody (orb1528041) and a second antibody against a different epitope of EHMT1.

Detection of human EHMT1 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-EHMT1 (orb1528041) used at a dilution of 1: 200 (1µg/ml). Detection: DAB