EHD1 Antibody

• Catalog Number: orb546323
• Category: Antibodies
• Description: EHD1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human EHD1 recombinant protein (Position: K324-E516).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 61 kDa
• UniProt ID: Q9H4M9
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: EH domain-containing protein 1; PAST homolog 1; hP
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U20S cells using anti-EHD1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of U87 cells using anti-EHD1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB:Lane 1:human placenta tissue;2:U87 cell;3:Caco-2 cell;4:rat testis tissue;5:rat testis tissue;6:rat kidney tissue;7:rat ovary tissue;8:mouse testis tissue;9:mouse testis tissue;10:mouse kidney tissue;11:mouse ovary tissue.

IF analysis of EHD1 using anti-EHD1 antibody.EHD1 was detected in immunocytochemical section of A431 cells.

IHC analysis of EHD1 using anti-EHD1 antibody.EHD1 was detected in paraffin-embedded section of human testis cancer tissues.

IHC analysis of EHD1 using anti-EHD1 antibody.EHD1 was detected in paraffin-embedded section of human tonsil tissues.

IHC analysis of EHD1 using anti-EHD1 antibody.EHD1 was detected in paraffin-embedded section of human placenta tissues.

IHC analysis of EHD1 using anti-EHD1 antibody.EHD1 was detected in paraffin-embedded section of mouse testis tissues.

IHC analysis of EHD1 using anti-EHD1 antibody.EHD1 was detected in paraffin-embedded section of rat testis tissues.