EGFR (phospho-Tyr992) antibody

• Catalog Number: orb43774
• Category: Antibodies
• Description: Mouse Monoclonal to EGFR (phospho-Tyr992).
• Clonality: Monoclonal
• Clone Number: EM-12
• Tested applications: FC, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Synthetic phospho-peptide covering sequence around tyrosine 992 of human EGFR.
• Concentration: 1 mg/ml
• Dilution range: Western blotting: Recommended dilution: 1 μg/ml; positive control: EGF-stimulated A431, negative control: non-stimulated A431. Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: EGFR (pY992)
• Entrez: 1956
• UniProt ID: P00533
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-EGFR (Phospho-Tyr992) antibody
• Note: For research use only
• Application notes: Western blotting: Recommended dilution: 1 μg/ml; positive control: EGF-stimulated A431, negative control: non-stimulated A431. Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.
• Expiration Date: 12 months from date of receipt.

Immunoprecipitation of EGFR from EGF-treated A431 cells by monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal mAb108 (D). The precipitates were immunoblotted with EM-12 or EM-13 antibody, and goat anti-mouse-HRP.

Immunoprecipitation of EGFR from EGF-treated A431 cells by phosphospecific monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal mAb108 (D). The precipitates were immunoblotted with a commercial anti-EGFR polyclonal and goat anti-rabbit-HRP.

Western blotting analysis of EGFR (phospho-Tyr992) by mouse monoclonal antibody EM-12 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).

Separation of EGF stimulated A431 cell suspension stained using anti-human EGFR (pY992) (EM-12) purified antibody (concentration in sample 1 µg/ml, GAM PE, red-filled) from EGF stimulated A431 cell suspension unstained by primary antibody (GAM PE, black-dashed) in flow cytometry analysis (intracellular staining).