EGF Receptor / EGFR Antibody (Extracellular domain)

• Catalog Number: orb1151568
• Category: Antibodies
• Description: Rabbit monoclonal antibody to EGF Receptor
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Purity: Protein A/G affinity
• Immunogen: Amin0 acids 25-645 from the extracellular domain of the human protein were used as the immunogen for the recombinant EGF Receptor antibody.
• UniProt ID: P00533
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the recombinant EGFR antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: EGFR/6390R
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Aliquot the recombinant EGFR antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Hazard Information: This recombinant EGFR antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human placental tissue with recombinant EGFR antibody (clone EGFR/6390R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC staining of FFPE human testis tissue with recombinant EGFR antibody (EGFR/6390R) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant EGFR antibody (clone EGFR/6390R) as confirmation of integrity and purity.