EGF protein

SKU: orb175996

Description

Recombinant Human Epidermal Growth Factor produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 6222 Dalton

Research Area

Cancer Biology

Key Properties

• Source: E.coli
• Biological Activity: The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml corresponding to a specific activity of 1 x 10,000,000 Units/mg
• Reactivity: Human
• Purification: Determined by SDS-PAGE
• Purity: Greater than 98.0%
• Protein Sequence: NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR

Storage & Handling

• Storage: Store at room temperature for up to 3 weeks. Store desiccated at -20°C. Avoid freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Lyophilized from a concentrated (1mg/ml) solution containing no additives
• Reconstitution: It is recommended to reconstitute the lyophilized Interleukin-17in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only