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    EEF2K Antibody

    Catalog Number: orb763120

    DispatchUsually dispatched within 5-10 working days
    $ 520.00
    Catalog Numberorb763120
    CategoryAntibodies
    DescriptionEEF2K Antibody
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsELISA, FC, ICC, IF, WB
    ReactivityHuman, Rat
    IsotypeRabbit IgG
    ImmunogenE.coli-derived human EEF2K recombinant protein (Position: K162-A719).
    ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
    Dilution rangeWestern blot, 0.25-0.5 μg/ml, Human, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry, 1-3 μg/1x106 cells, Human Direct ELISA, 0.1-0.5 μg/ml, Human
    Form/AppearanceLyophilized
    ConjugationUnconjugated
    MW82 kDa
    UniProt IDO00418
    StorageAt -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
    NoteFor research use only
    Application notesTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
    Expiration Date12 months from date of receipt.
    EEF2K Antibody

    Western blot analysis of EEF2K using anti-EEF2K antibody (orb763120). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EEF2K antigen affinity purified polyclonal antibody (Catalog # orb763120) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for EEF2K at approximately 82 kDa. The expected band size for EEF2K is at 82 kDa.

    EEF2K Antibody

    IF analysis of EEF2K using anti-EEF2K antibody (orb763120). EEF2K was detected in an immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (orb90553) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-EEF2K Antibody (orb763120) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

    EEF2K Antibody

    Flow Cytometry analysis of SiHa cells using anti-EEF2K antibody (orb763120). Overlay histogram showing SiHa cells stained with orb763120 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EEF2K Antibody (orb763120, 1 μg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 μg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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      ELISA,  IHC,  WB

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      Polyclonal

      Unconjugated

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