EEF1B2 Antibody

• Catalog Number: orb1265033
• Category: Antibodies
• Description: EEF1B2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Gallus
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This EEF1B2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 54-86 amino acids from the Central region of human EEF1B2.
• Concentration: batch dependent
• Dilution range: For FACS starting dilution is: 1:25For IF starting dilution is: 1:25For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 25 kDa
• Target: EEF1B2
• UniProt ID: P24534
• NCBI: P24534
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Elongation factor 1-beta, EF-1-beta, EEF1B2, EEF1B
• Note: For research use only
• Application notes: For FACS starting dilution is: 1:25For IF starting dilution is: 1:25For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of Hela cells using EEF1B2 Antibody (green) compared to an isotype control of rabbit IgG (blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

Fluorescent image of Hela cells stained with EEF1B2 Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Immunohistochemical analysis of paraffin-embedded H. brain section using EEF1B2 Antibody. Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from Hela, K562 cell line (from left to right), using EEF1B2 Antibody at 1:1000 at each lane.