EDG3 antibody

• Catalog Number: orb100194
• Category: Tools
• Description: EDG3 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Bovine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human EDG3 (145-250/378 aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, Flow-Cyt=0.2u/test, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 42 kDa
• Target: EDG3
• UniProt ID: Q99500
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: EDG 3; EDG3; Endothelial differentiation G protein
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of mouse heart lysates (Lane1), mouse liver lysates (Lane2) dilution at:1:200 using EDG3 antibody

Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat