EDG1 antibody

• Catalog Number: orb100426
• Category: Antibodies
• Description: EDG1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Predicted Reactivity: Canine, Equine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human EDG1/CD363 (51-150/382 aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 44 kDa
• Target: EDG1
• UniProt ID: P21453
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: CD363; CHEDG1; D1S3362; ECGF1; EDG1; EDG 1; Endoth
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Korbelik, Mladen et al. Ceramide and sphingosine-1-phosphate act as photodynamic therapy-elicited damage-associated molecular patterns: cell surface exposure Int Immunopharmacol, 20, 359-365 (2014)

PubMed: 24713544

Paraffin-embedded human lung carcinoma fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.). EDG1 antibody at 1:200 dilution with overnight incubation at 4 degrees