EDA Antibody

• Catalog Number: orb1239848
• Category: Antibodies
• Description: EDA Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: EDA1 antibody was raised against an 19 amino acid peptide near the center of human EDA1.The immunogen is located within amino acids 130 - 180 of EDA1.
• Concentration: 1 mg/mL
• Dilution range: EDA1 antibody can be used for detection of EDA1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 16, 43 kDa Observed: 39 kDa
• Target: EDA
• UniProt ID: Q92838
• NCBI: NP_001390
• Storage: EDA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
• Buffer/Preservatives: EDA1 antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Ectodysplasin A, Ectodermal dysplasia protein, ED1
• Note: For research use only
• Application notes: EDA1 antibody can be used for detection of EDA1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of EDA1 in mouse kidney tissue lysate with EDA1 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry of EDA1 in mouse kidney tissue with EDA1 antibody at 5 µg/ml.

Immunofluorescence of EDA1 in mouse kidney tissue with EDA1 antibody at 20 µg/ml.