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ECS (DYKDDDDK) Tag Antibody

Catalog Number: orb1530835

DispatchUsually dispatched within 5-10 working days
$ 471.00
Catalog Numberorb1530835
CategoryAntibodies
DescriptionECS (DYKDDDDK) Tag Antibody
Species/HostRabbit
ClonalityRecombinant
Tested applicationsELISA, ICC, IP, WB
ImmunogenEnterokinase Cleavage Site (DYKDDDDK)
Concentration100 µg/ml
Dilution rangeWB - 1:1000; IP - 20 µl/mg lysate; ICC - 1:1000 to 1:5000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; ELISA - 1:1,000 - 1:75,000; for coating plates 1:100 - 1:500
Form/AppearanceWhole IgG
ConjugationUnconjugated
TargetECS (DYKDDDDK) Tag
Storage2 - 8°C
Buffer/PreservativesBorate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
NoteFor research use only
Expiration Date12 months from date of receipt.

Detection of ECS (DYKDDDDK)-tagged protein by WB from non-transfected HEK293 cells, HEK293 transfected with Met-ECS(DYKDDDDK)-IDO1, IDO1-ECS(DYKDDDDK)-HIS (Internal Tag), IDO1-HIS-ECS(DYKDDDDK) (C-terminal Tag), and SUP-M2 cells, at 1:1000.

Detection of ECS (DYKDDDDK) Tag by western blot of immunoprecipitates from HEK293 transfected with IDO1-HIS-ECS(DYKDDDDK) (C-terminal Tag) lysate.Antibody: Rabbit anti-ECS (DYKDDDDK) Tag recombinant monoclonal antibody.

Detection of ECS (DYKDDDDK) Tag by western blot of immunoprecipitates from HEK293 transfected with Met-ECS(DYKDDDDK)-IDO1 lysate.Antibody: Rabbit anti-ECS (DYKDDDDK) Tag recombinant monoclonal antibody.

Detection of ECS (DYKDDDDK) Tag in FFPE HEK293 cells expressing ECS (DYKDDDDK)-tagged nuclear protein by ICC.Antibody: Rabbit anti-ECS (DYKDDDDK) Tag recombinant monoclonal antibody.

Detection of ECS (DYKDDDDK) Tag in FFPE HEK293 cells expressing an ECS (DYKDDDDK)-tagged cytoplasmic protein by ICC.Antibody: Rabbit anti-ECS (DYKDDDDK) Tag recombinant monoclonal antibody.

  • FLAG Tag (CT) antibody [orb612171]

    WB

    All

    Rabbit

    Polyclonal

    Unconjugated

    500 μl, 50 μl, 100 μl
  • ECS (DYKDDDDK) Tag Antibody [orb1530884]

    ELISA,  ICC,  IP,  WB

    Goat

    Polyclonal

    Unconjugated

    0.1 mg
  • Detection of FLAG antibody [orb344415]

    ELISA,  FC,  IHC,  WB

    Mouse

    Monoclonal

    Unconjugated

    25 μl
  • Flag Tag antibody [orb526695]

    ELISA,  IF,  WB

    All

    Mouse

    Monoclonal

    Unconjugated

    100 μl, 500 μl, 50 μl
  • FLAG Tag antibody [orb312242]

    ELISA,  WB

    All

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 200 μl, 50 μl
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