Ebolavirus EBOV (subtype Zaire, strain Kikwit-95) Envelope Glycoprotein (GP) Protein

• Catalog Number: orb257934
• Category: Proteins
• Description: EBOV encodes seven structural proteins: nucleoprotein (NP), polymerase cofactor (VP35), (VP40), GP, transcription activator (VP30), VP24, and RNA polymerase (L). GP protein contains 160-kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP). GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . GP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. GP2 acts as a class I viral fusion protein. GP1,2 mediates endothelial cell activation and decreases endothelial barrier function. sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha.
• Reactivity: Virus
• Tag: C-7×His
• Form/Appearance: Powder
• Purity: 85%
• Conjugation: Unconjugated
• MW: 67.2 kDa
• Target: Glycoprotein / GP (virus)
• Protein Sequence: AAQ55048.1
• Source: Ebolavirus EBOV (subtype Zaire, strain Kikwit-95) GP, His Tag (orb257934) is expressed from human 293 cells (HEK293). It contains AA Ile 33 - Asp 637 (Accession # AAQ55048.1).
• Expression System: HEK293
• Expression Region: Ile 33 - Asp 637
• Endotoxins: 1.0 EU per μg
• NCBI: AAQ55048.1
• Storage: -20℃
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: GP,Envelope glycoprotein,GP(1,2)
• Note: For research use only
• Application notes: Ebolavirus EBOV (subtype Zaire, strain Kikwit-95) GP, His Tag, containing GP1 and GP2, is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 67.2 kDa. The reducing (R) protein migrates as 21-23 kDa (GP2-delta) and 110-120 kDa (GP1) in SDS-PAGE.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Viral Glycoprotein protein