EBI3 Antibody / Epstein-Barr virus induced 3

• Catalog Number: orb1824783
• Category: Antibodies
• Description: Epstein-Barr virus-induced gene 3 (Ebi3) is a widely expressed homolog to the interleukin IL-12 p40 subunit protein that forms a heterodimer with either IL-12 p35 or an IL-12 p35 homolog, p28, to create a new cytokine (IL-27). IL-27 is an early product of activated antigen-presenting cells and drives rapid clonal expansion of naive but not memory CD4+ T cells. Interferon-b differentially regulates expression of the IL-12 family members p35, p40, p19 and Ebi3 in activated human dendritic cells. Ebi3 may function to antagonize IL-12 and to inhibit the development of a Th1 immune response. Ebi3 is strongly expressed in Hodgkin and Reed-Sternberg cells, independently of the EBV status of the tumor cells. Research suggests that Ebi3 may be an additional component of the repertoire employed by Hodgkin and Reed-Sternberg cells to inhibit an effective anti-tumor or anti-viral immune response. The human Ebi3 gene maps to chromosome 19p13.3 and encodes a secreted glycoprotein that is expressed in spleen and tonsils, and at high levels in full-term placenta.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: EBI3/8902
• Tested applications: ELISA, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A recombinant partial protein sequence (within amino acids 366-466) from the human protein was used as the immunogen for the EBI3 antibody.
• Dilution range: ELISA (Order BSA-free format for coating),Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This EBI3 antibody is available for research use only.
• UniProt ID: Q14213
• Storage: Aliquot the EBI3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.