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Catalog Number | orb176694 |
---|---|
Category | Antibodies |
Description | E3 SUMO-protein ligase PIAS1 PIAS1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ICC, IHC, WB |
Predicted Reactivity | Hamster |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human PIAS1 (636-651aa DTASIFGIIPDIISLD), identical to the related mouse and rat sequences. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 71836 MW |
UniProt ID | O75925 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | E3 SUMO-protein ligase PIAS1;6.3.2.-;DEAD/H box-bi Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Confocal immunofluoroscence analysis of A549 cells using PIAS1 antibody
Western blot analysis of Lane 1: Rat Testis Tissue Lysate, Lane 2: Mouse Testis Tissue Lysate, Lane 3: HELA Whole Cell Lysate, Lane 4: JURKAT Whole Cell Lysate, Lane 5: MCF-7 Whole Cell Lysate, Lane 6: SKOV Whole Cell Lysate using PIAS1 antibody
Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Testis Tissue using PIAS1 antibody
Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Brain Tissue using PIAS1 antibody
Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Brain Tissue using PIAS1 antibody
Immunohistochemical analysis of formalin-fixed paraffin embedded Human Placenta Tissue using PIAS1 antibody
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, ICC, IF, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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