E2F8 Antibody

• Catalog Number: orb1525566
• Category: Antibodies
• Description: E2F8 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: between 817 and 867
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: E2F8
• UniProt ID: A0AVK6
• NCBI: NP_078956.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: E2F family member 8; E2F-8; transcription factor E
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human E2F8 by western blot. Samples: Whole cell lysate (10 µg) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-E2F8 antibody orb1525566 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human E2F8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-E2F8 antibody orb1525566 used for IP at 6 µg per reaction.