E1(Estrone) ELISA Kit

• Catalog Number: orb1806511
• Category: Assays and Kits
• Description: E1(Estrone) ELISA Kit
• Reactivity: All
• Range: 31.25-2000 pg/mL
• Target: E1
• Sensitivity: 8.86 pg/mL
• Alternative names: Oestrone
• Note: For research use only
• Application notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Estrone(E1) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Estrone(E1). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Estrone(E1) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Sample Types: Serum, plasma and other biological fluids
• Expiration Date: Please enquire.