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Catalog Number | orb234322 |
---|---|
Category | Antibodies |
Description | Dystrophin/DMD Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IHC, IHC-Fr, WB |
Predicted Reactivity | Bovine, Canine, Gallus, Monkey, Rabbit |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human Dystrophin recombinant protein (Position: H3076-D3404). Human Dystrophin shares 100% amino acid (aa) sequence identity with mouse Dystrophin. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry, 0.5-1μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 426750 MW |
UniProt ID | P11532 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Dystrophin;DMD; Read more... |
Note | For research use only |
Application notes | WB: The detection limit for Dystrophin is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of HepG2 cells using anti-Dystrophin antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of Dystrophin using anti-Dystrophin antibody.Lane 1:human HEK293 cell;2:human K562 cell;3:mouse HEPA1-6 cell.
IHC analysis of Dystrophin using anti-Dystrophin antibody.Dystrophin was detected in paraffin-embedded section of Mouse Brain Tissue.
IHC analysis of Dystrophin using anti-Dystrophin antibody.Dystrophin was detected in paraffin-embedded section of Rat Cardiac Muscle Tissue.
IHC analysis of Dystrophin using anti-Dystrophin antibody.Dystrophin was detected in paraffin-embedded section of Human Lung Cancer Tissue.
IHC, WB | |
Human, Mouse, Rabbit, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
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