DYKDDDDK (FLAG tag) antibody (Agarose)

• Catalog Number: orb344560
• Category: Antibodies
• Description: Mouse monoclonal antibody against DYKDDDDK (FLAG tag) conjugated to Agarose.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 29E4.G7
• Tested applications: IP, WB
• Isotype: IgG2a
• Immunogen: Anti-DYKDDDDK Affinity Gel antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH.
• Concentration: 0.99 mg
• Dilution range: IP: 10 µL resin binds >1 µg FLAG fusion protein
• Form/Appearance: Suspension of agarose beads
• Purity: Anti-DYKDDDDK Affinity Gel is a purified mouse IgG2a monoclonal antibody coupled to activated agarose. This product is intended for purification of proteins containing the FLAG epitope tag sequence. Binding Specificity: Anti-DYKDDDDK Affinity Gel binds the FLAG epitope tag sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to the amino terminal, carboxy terminal or internal locations of targeted recombinant proteins expressed in transfected or transformed cells. D-Y-K-D-D-D-D-K peptide (p/n 000-000-383) is recommended for competitive elution to recover fusion protein (see protocol).
• Conjugation: Agarose
• Storage: Store vial at 4°C prior to opening.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse anti-FLAG tag agarose gel, mouse anti-DYKDDD
• Note: For research use only
• Application notes: Anti-DYKDDDDK Affinity Gel has been tested by SDS-Page, IP, and western blot and is optimally suited for immunoprecipitation and purification of FLAG tagged fusion proteins. Anti-DYKDDDDK Affinity Gel antibody recognizes the FLAG epitope tag fused to either the amino- or carboxy- terminal ends or an internal location of targeted fusion proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag. Use D-Y-K-D-D-D-D-K peptide (p/n 000-000-383) for competitive elution to recover fusion protein (see protocol). Anti-FLAG is a registered trademark of Sigma-Aldrich. Refer to the Protocol for complete instructions for use including preferred buffers for elution. Do not use buffers that may denature the anti-DYKDDDDK antibody.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of DYKDDDDK tagged recombinant protein flow through wash eluted fraction using DYKDDDDK (FLAG tag) antibody (Agarose)

Western blot analysis of crude lysate containing over-expressed DYKDDDK-tagged recombinant protein using DYKDDDDK (FLAG tag) antibody (Agarose)

Western blot analysis of Cell lysate before purification using DYKDDDDK (FLAG tag) antibody (Agarose)