DUX4 Antibody

SKU: orb67380

Description

Mouse Anti-Human DUX4 Monoclonal IgG1

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution Range: WB (1:1000), ICC/IF (1:1000)
• Reactivity: Human, Mouse
• Application Notes:
  1 µg/ml of SMC-192 was sufficient for detection of DUX4 in 20 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-mouse IgG: HRP as teh secondary.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: P2B1
• Immunogen: C-terminal 76 amino acids of DUX4 with glutathione-s-transferase (gst) tag
• Target: DUX4
• Molecular Weight: 45kDa
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Double homeobox 4, Double homeobox 10, DUX10, Double homeobox protein 4/10, DUX4L, DUX4L1

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-DUX4 Monoclonal Antibody, Clone P2B1. Tissue: C2C12 myoblast cells. Species: Mouse. Primary Antibody: Mouse Anti-DUX4 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain. Cells transfected with pCS2+DUX4.

Western Blot analysis of Mouse C2C12 cell lysate showing detection of DUX4 protein using Mouse Anti-DUX4 Monoclonal Antibody, Clone P2B1. Primary Antibody: Mouse Anti-DUX4 Monoclonal Antibody at 1:1000. Cells transfected with pCS2+DUX4 which, contains an additional upstream start site.

Quick Database Links

Gene Symbol

DUX4

UniProt

F5GZ66

NCBI Gene ID

22947

RefSeq (Protein):NP_149418.3