dUTPase/DUT Antibody

• Catalog Number: orb1248032
• Category: Antibodies
• Description: dUTPase/DUT Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Porcine
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-QLRFARLSEHATAPT
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 20kDa band observed in lysates of cell lines HEK293 and HeLa (calculated MW of 17.7kDa according to NP_001939.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Testis and Tonsil. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 20kDa
• Target: dUTPase / DUT
• UniProt ID: P33316
• NCBI: NP_001020420.1, NP_001939.1, NP_001020419.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: DUT, deoxyuridine triphosphatase, dUTPase, dUTP nu
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 20kDa band observed in lysates of cell lines HEK293 and HeLa (calculated MW of 17.7kDa according to NP_001939.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Testis and Tonsil. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248032 (0.1 ug/ml) staining of HEK293 (A) and HeLa (B) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248032 (5 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1248032 (5 ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.