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Catalog Number | orb533050 |
---|---|
Category | Small Molecules |
Description | dUTP, PCR-grade is supplied as ultrapure aqueous solution (pH 8.5) and suitable for all molecular biology applications including PCR/qPCR, reverse transcription, DNA labeling and DNA sequencing. |
Form/Appearance | clear aqueous solution; pH: 8.5 ± 0.2 (22 °C) |
Concentration | 100 mM-110 mM |
Purity | ≥ 99% (HPLC) |
MW | Theoretical MW: 468.14 g/mol (free acid) |
Application notes | Spectroscopic Propertie: λmax 262 nm, ε 9.8 L mmol-1 cm-1 (Tris-HCl, pH 7.0). |
CAS Number | 102814-08-4 |
Formula | C9H15N2O14P3 |
SMILES | O=c1ccn(c(=O)[nH]1)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)C1 |
Storage | store at -20 °C |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
≥ 95% (HPLC) | |
116840-18-7 | |
Theoretical MW: 523.22 g/mol (free acid); Detected MW: 523.02 g/mol (free acid) | |
C12H20N3O14P3 |
≥ 95% (HPLC) | |
116840-18-7 | |
Theoretical MW: 523.22 g/mol (free acid); Detected MW: 523.02 g/mol (free acid) | |
C12H20N3O14P3 |
≥ 95% (HPLC) | |
116840-18-7 | |
Theoretical MW: 523.22 g/mol (free acid); Detected MW: 523.02 g/mol (free acid) | |
C12H20N3O14P3 |
≥ 99% (HPLC) | |
Theoretical MW: dATP: 491.18 g/mol (free acid), dCTP: 467.15 g/mol (free acid), dGTP: 507.18 g/mol (free acid), dUTP: 468.14 g/mol (free acid) | |
dATP: C 10H16N5O12P3; dCTP: C 9H16N3O13P3; dGTP: C 10H16N5O13P3; dUTP: C 9H15N2O14P3 |
For example, if the dosage of your experiment is 10mg/kg, the weight of each animal is 50g, and the volume of the dosing is 100μL, then the working solution concentration of your experiment is 5mg/mL. If the formula you used is 5% DMSO+ 30% PEG300+ 5% Tween 80 + 60% Saline/PBS/ddH2O, the preparation method is as follows:
Mother liquor preparation method: 5mg of drug dissolved in 100μL DMSO (mother liquor concentration is 50mg/mL). Preparation method of in vivo formulation: For 1mL of working solution preparation, take 50μL of DMSO mother liquor, add 300 μL of PEG300 to mix well and clarify, then add 50μL of Tween-80 to mix well and clarify, and finally add 600μL of Saline/PBS/ddH2O to mix well and clarify.