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Item 1 of 6
Item 1 of 6
DUSP6 monoclonal antibody (M01), clone 3G2
Catalog Number: orb2293437
| Catalog Number | orb2293437 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody raised against a full length recombinant DUSP6. |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | 3G2 |
| Tested applications | ELISA, IF, IHC-P, WB |
| Reactivity | Human |
| Isotype | IgG1 Kappa |
| Immunogen | DUSP6 (AAH03143, 1 a.a. ~ 381 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Conjugation | Unconjugated |
| Protein Sequence | MIDTLRPVPFASEMAISKTVAWLNEQLELGNERLLLMDCRPQELYESSHIESAINVAIPGIMLRRLQKGNLPVRALFTRGEDRDRFTRRCGTDTVVLYDESSSDWNENTGGESLLGLLLKKLKDEGCRAFYLEGGFSKFQAEFSLHCETNLDGSCSSSSPPLPVLGLGGLRISSDSSSDIESDLDRDPNSATDSDGSPLSNSQPSFPVEILPFLYLGCAKDSTNLDVLEEFGIKYILNVTPNLPNLFENAGEFKYKQIPISDHWSQNLSQFFPEAISFIDEARGKNCGVLVHCLAGISRSVTVTVAYLMQKLNLSMNDAYDIVKMKKSNISPNFNFMGQLLDFERTLGLSSPCDNRVPAQQLYFTTPSNQNVYQVDSLQST |
| NCBI | AAH03143 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | In 1x PBS, pH 7.4 |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Detection limit for recombinant GST tagged DUSP6 is approximately 1 ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to DUSP6 on HeLa cell. [antibody concentration 10 ug/ml]
Immunoperoxidase of monoclonal antibody to DUSP6 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 ug/ml]
Western Blot analysis of DUSP6 expression in transfected 293T cell line by DUSP6 monoclonal antibody (M01), clone 3G2. Lane 1: DUSP6 transfected lysate (42 KDa). Lane 2: Non-transfected lysate.
Western blot analysis of DUSP6 over-expressed 293 cell line, cotransfected with DUSP6 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with DUSP6 monoclonal antibody (M01), clone 3G2 (Cat # orb2293437). GAPDH (36.1 kDa) used as specificity and loading control.
Western Blot detection against Immunogen (67.65 KDa).
