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dTTP - Solution
Catalog Number: orb533047
| Catalog Number | orb533047 |
|---|---|
| Category | Small Molecules |
| Description | dTTP, PCR-grade is supplied as ultrapure aqueous solution (pH 8.5) and suitable for all molecular biology applications including PCR/qPCR, reverse transcription, DNA labeling and DNA sequencing. |
| Form/Appearance | clear aqueous solution; pH: 8.5 ± 0.2 (22 °C) |
| Concentration | 100 mM-110 mM |
| Purity | ≥ 99% (HPLC) |
| MW | Theoretical MW: 482.17 g/mol (free acid) |
| Application notes | Spectroscopic Propertie: λmax 267 nm, ε 9.5 L mmol-1 cm-1 (Tris-HCl, pH 7.0). |
| CAS Number | 18423-43-3 |
| Formula | C10H17N2O14P3 |
| SMILES | Cc1cn(c(=O)[nH]c1=O)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)C1 |
| Storage | store at -20 °C |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
dTTP - Solution [orb1734228]
≥ 99% (HPLC)
18423-43-3
Theoretical MW: 482.17 g/mol (free acid)
C10H17N2O14P3
1 ml
For example, if the dosage of your experiment is 10mg/kg, the weight of each animal is 50g, and the volume of the dosing is 100μL, then the working solution concentration of your experiment is 5mg/mL. If the formula you used is 5% DMSO+ 30% PEG300+ 5% Tween 80 + 60% Saline/PBS/ddH2O, the preparation method is as follows:
Mother liquor preparation method: 5mg of drug dissolved in 100μL DMSO (mother liquor concentration is 50mg/mL). Preparation method of in vivo formulation: For 1mL of working solution preparation, take 50μL of DMSO mother liquor, add 300 μL of PEG300 to mix well and clarify, then add 50μL of Tween-80 to mix well and clarify, and finally add 600μL of Saline/PBS/ddH2O to mix well and clarify.Note:
- For Reference Only. Please choose the appropriate dissolution protocol according to your experimental animal and administration method.
- We suggest that in cell experiments, the final concentration of DMSO is not more than 0.1%, in animal experiments, for healthy mice, the final concentration of DMSO is not more than 10%; For nude or frail mice, the final concentration of DMSO is not more than 2%.
