DSG3 Antibody

• Catalog Number: orb1252262
• Category: Antibodies
• Description: DSG3 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: DSG3/1535
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: A protein fragment corresponding to the extracellular portion of human DSG3 was used as the immunogen for the Desmoglein 3 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: WB: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RTThe stated application concentrations are suggested starting amounts. Titration of the Desmoglein 3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: DSG3
• UniProt ID: P32926
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Note: For research use only
• Application notes: WB: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RTThe stated application concentrations are suggested starting amounts. Titration of the Desmoglein 3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human skin with Desmoglein 3 antibody (clone DSG3/1535). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

IHC testing of FFPE human lung squamos cell carcinoma with Desmoglein 3 antibody (clone DSG3/1535). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.