DRAM1 Antibody

• Catalog Number: orb1239371
• Category: Antibodies
• Description: DRAM1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-DRAM antibody (orb1239371) was raised against a peptide corresponding to 16 amino acids near the amino terminus of human DRAM. The immunogen is located within amino acids 30-80 of DRAM.
• Concentration: 1 mg/mL
• Dilution range: WB: 1-2 μg/mL; IF: 20 μg/mL; IHC: 2-2.5 μg/mL.Antibody validated: Western Blot in human mouse and rat samples; Immunofluorescence and Immunohistochemistry in human and mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 14kD, 26kDObserved: 14 kD, 26kD
• Target: DRAM1
• UniProt ID: Q8N682
• NCBI: AAH18435
• Storage: DRAM antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: DRAM Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: DRAM Antibody: DRAM, DRAM, DNA damage-regulated au
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Human 293 Cell Lysate. Loading: 15 µg of lysate per lane. Antibodies: DRAM orb1239371 (A: 1 µg/mL, B: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: DRAM orb1239379 (0.5 µg/mL), DRAM orb1239371 (2 µg/mL), beta-actin (1 µg/mL) and GAPDH (0.02 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunofluorescence Validation of DRAM in Human Liver Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human liver tissue labeling DRAM with orb1239371 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Immunohistochemistry Validation of DRAM in Human Liver Tissue. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-DRAM antibody (orb1239371) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of DRAM in Mouse Liver Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse liver tissue labeling DRAM with orb1239371 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).

Immunohistochemistry Validation of DRAM in Mouse Liver Tissue. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-DRAM antibody (orb1239371) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.