DPH2 monoclonal antibody (M07), clone 3F7

• Catalog Number: orb2293510
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full-length recombinant DPH2.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3F7
• Tested applications: ELISA, IF, WB
• Reactivity: Human
• Isotype: IgG2a Kappa
• Immunogen: DPH2 (AAH16956, 1 a.a. ~ 489 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: MESMFSSPAEAALQRETGVPGLLTPLPDLDGVYELERVAGFVRDLGCERVALQFPDQLLGDAVAVAARLEETTGSKMFILGDTAYGSCCVDVLGAEQAGAQALIHFGPACLSPPARPLPVAFVLRQRSVALELCVKAFEAQNPDPKAPVVLLSEPACAHALEALATLLRPRYLDLLVSSPAFPQPVGSLSPEPMPLERFGRRFPLAPGRRLEEYGAFYVGGSKASPDPDLDPDLSRLLLGWAPGQPFSSCCPDTGKTQDEGARAGRLRARRRYLVERARDARVVGLLAGTLGVAQHREALAHLRNLTQAAGKRSYVLALGRPTPAKLANFPEVDVFVLLACPLGALAPQLSGSFFQPILAPCELEAACNPAWPPPGLAPHLTHYADLLPGSPFHVALPPPESELWETPDVSLITGDLRPPPAWKSSNDHGSLALTPRPQLELAESSPAASFLSSRSWQGLEPRLGQTPVTEAVSGRRGIAIAYEDEGSG
• NCBI: AAH16956
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged DPH2 is 3 ng/ml as a capture antibody.

Immunofluorescence of monoclonal antibody to DPH2 on HeLa cell. [antibody concentration 10 ug/ml]

Western Blot analysis of DPH2 expression in transfected 293T cell line by DPH2 monoclonal antibody (M07), clone 3F7. Lane 1: DPH2 transfected lysate (Predicted MW: 52.1 KDa). Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (79.53 KDa).