Double Stranded DNA Antibody [SPM603]

SKU: orb1252400

Description

Double Stranded DNA Antibody [SPM603]

Images & Validation

• Tested Applications: FC, ICC, IF, IHC-P
• Reactivity: Human
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 µg/mLImmunocytochemistry (Acetone-fixed): 0.5-1 µg/mL for 30 min at RTImmunohistochemistry (FFPE): 1-2 µg/mL for 30 min at RT (1)Optimal dilution of the Double Stranded DNA antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG3, kappa
• Clone No.: SPM603
• Immunogen: Nuclei of Burkitt's cells were used as the immunogen for the Double Stranded DNA antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Formalin-fixed, paraffin-embedded human tonsil stained with Double Stranded DNA antibody (SPM603)

Flow cytometry testing of fixed and permeabilized human Jurkat cells with dsDNA antibody (clone SPM603); Red = isotype control, Blue = dsDNA antibody.

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UniProt

NotKnown