You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1252400 |
---|---|
Category | Antibodies |
Description | Double Stranded DNA Antibody [SPM603] |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | SPM603 |
Tested applications | FC, ICC, IF, IHC-P |
Reactivity | Human |
Isotype | IgG3, kappa |
Immunogen | Nuclei of Burkitt's cells were used as the immunogen for the Double Stranded DNA antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunocytochemistry (Acetone-fixed): 0.5-1 ug/ml for 30 min at RTImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)Optimal dilution of the Double Stranded DNA antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
UniProt ID | NotKnown |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Note | For research use only |
Application notes | Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunocytochemistry (Acetone-fixed): 0.5-1 ug/ml for 30 min at RTImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)Optimal dilution of the Double Stranded DNA antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
Formalin-fixed, paraffin-embedded human tonsil stained with Double Stranded DNA antibody (SPM603)
Flow cytometry testing of fixed and permeabilized human Jurkat cells with dsDNA antibody (clone SPM603); Red = isotype control, Blue = dsDNA antibody.
FC, ICC, IF, IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
Filter by Rating