DOTAP chloride

SKU: orb1309745

Description

DOTAP chloride (1,2-Dioleoyl-3-trimethylammonium-propane chloride) is a useful and effective cationic lipid transfection DNA(plasmids, BacMIDs) and modified nucleic acids (antisense oligonucleotides) without the use of auxiliary lipids.

Research Area

Metabolism Research

Key Properties

• CAS Number: 132172-61-3
• MW: 698.54
• Purity: 99.97% (May vary between batches)
• Formula: C₄₂H₈₀ClNO₄
• SMILES: [Cl-].CCCCCCCC\C=C\CCCCCCCC(=O)OC[C@H](C[N+](C)(C)C)OC(=O)CCCCCCC\C=C\CCCCCCCC
• Target: Others
• Solubility: Ethanol: 130 mg/mL (186.1 mM), Sonication and heating are recommended. DMSO: 125 mg/mL (178.94 mM), Sonication is recommended.

Bioactivity

• Target IC50:
  293T cells:58.6 µM
• In Vitro:
  METHODS: Prostate cancer cell line PC3 and melanoma cell line M21 were treated with paclitaxel (PTX) loaded on DOTAP/DOPC liposome carriers (CLs) for 24 hours. The cytotoxicity was detected by measuring the absorbance at 490 nm using an enzyme-linked immunosorbent assay reader. RESULTS: With the increase of PTX content, the cytotoxic effect of DOTAP/DOPC CLs is enhanced.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Lipid, Inhibitor, inhibit, nucleic, DOTAP, DOTAP chloride, DOTAP Chloride, Cationic Lipid, Cationic, acid, 1,2-Dioleoyl-3-trimethylammonium-propane Chloride, 1,2-Dioleoyl-3-trimethylammonium-propane chloride, transfection

Compound Identifiers

PubChem CID

129009514