DOTAP chloride

SKU: orb1309745

Description

DOTAP chloride (1, 2-dioleoyl-3-trimethylammonium-propane chloride) is an efficient cationic lipid, and its target is mainly the negatively charged components on the cell membrane. DOTAP chloride binds to negatively charged nucleic acids (such as DNA and RNA) by electrostatic interaction to form complexes, thereby achieving the delivery of nucleic acids.

Research Area

Metabolism Research

Key Properties

• CAS Number: 132172-61-3
• MW: 698.54
• Purity: >99.99% (May vary between batches)
• Formula: C₄₂H₈₀ClNO₄
• SMILES: [Cl-].CCCCCCCC\C=C\CCCCCCCC(=O)OC[C@H](C[N+](C)(C)C)OC(=O)CCCCCCC\C=C\CCCCCCCC
• Target: Others,Liposome
• Solubility: Ethanol: 130 mg/mL (186.1 mM), Sonication and heating are recommended. DMSO: 125 mg/mL (178.94 mM), Sonication is recommended.

Bioactivity

• Target IC50:
  293T cells:58.6 µM
• In Vitro:
  METHODS: Prostate cancer cell line PC3 and melanoma cell line M21 were treated with paclitaxel (PTX) loaded on DOTAP/DOPC liposome carriers (CLs) for 24 hours. The cytotoxicity was detected by measuring the absorbance at 490 nm using an enzyme-linked immunosorbent assay reader. RESULTS: With the increase of PTX content, the cytotoxic effect of DOTAP/DOPC CLs is enhanced.

Storage & Handling

• Storage: store at low temperature | Pure form: -20°C for 3 years | In solvent: -80°C for 1 year
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Lipid, Inhibitor, inhibit, nucleic, DOTAP, DOTAP chloride, DOTAP Chloride, Cationic Lipid, Cationic, acid, 1,2-Dioleoyl-3-trimethylammonium-propane Chloride, 1,2-Dioleoyl-3-trimethylammonium-propane chloride, transfection

Compound Identifiers

PubChem CID

129009514