DOT1L Antibody

• Catalog Number: orb1529334
• Category: Antibodies
• Description: DOT1L Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, IHC, IP, WB
• Reactivity: Human
• Immunogen: between 1000 and 1050
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: DOT1L
• UniProt ID: Q8TEK3
• NCBI: NP_115871.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: H3-K79-HMTase; histone H3-K79 methyltransferase; l
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human DOT1L by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-DOT1L used at a dilution of 1:200 (1µg/ml).

Detection of human DOT1L by WB and IP.Samples: HeLa cells. Antibodies: Affinity purified rabbit anti-DOT1L antibody orb1529334 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B).

Localization of DOT1L Binding Sites by ChIP-sequencing. Chromatin from KOPN-8 cell line was immunoprecipitated with anti-DOT1L antibody and analyzed by DNA sequencing.