DOK5 antibody

• Catalog Number: orb18915
• Category: Antibodies
• Description: Goat polyclonal to DOK5
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:16000, WB: 1-3 μg/ml, IHC-P: 1-2µg/ml
• Conjugation: Unconjugated
• MW: 35.5
• Target: DOK5
• Entrez: 55816
• Protein Sequence: CLHRTETFPAYRSEH
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Alternative names: anti DOK5 antibody, anti MGC16926 antibody, anti C
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded tonsil, stains scattered cells in the interfollicular area compatible with mast cells. Recommended concentration, 1-2ug/ml.WB: In transfected HEK293 transiently expressing DOK5 a band of approx. 38kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 35.5kDa according to NP_060901.2. Recommended concentration, 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

HEK293 overexpressing DOK5 (RC211718) and probed with orb18915 (mock transfection in first lane), tested by Origene.

orb18915 (1ug/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with citrate buffer pH6, HRP-staining.