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Dog IntactParathormone (I-PTH) ELISA Kit

Catalog Number: orb779887

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb779887
CategoryAssays and Kits
DescriptionThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Parathyroid Hormone(PTH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Parathyroid Hormone(PTH).
Tested applicationsELISA
ReactivityCanine
Range12.5-800 pg/mL
Assay Time3.5h
Concentration800 pg/mL
Sensitivity3.98 pg/mL
Alternative namesiPTH ELISA Kit, Intact Parathyroid Hormone ELISA K
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NoteFor research use only
Application notesstandard: 800 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog I-PTH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog I-PTH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog I-PTH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog I-PTH in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Typesserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Expiration DatePlease enquire.
Dog IntactParathormone (I-PTH) ELISA Kit