Dog Bone-specific Alkaline Phosphatase (BALP) ELISA Kit

• Catalog Number: orb1146803
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone-specific alkaline phosphatase(BALP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Bone-specific alkaline phosphatase(BALP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone-specific alkaline phosphatase(BALP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bone-specific alkaline phosphatase(BALP) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Canine
• Concentration: 20 ng/mL
• Application notes: standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog BALP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog BALP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog BALP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog BALP in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.112 ng/mL
• Sample Types: serum, plasma and other biological fluids
• Alternative names: BALP, BSAP, HOPS, AP-TNAP, TNSALP, Bone Alkaline P
• Research Area: Gastroenterology & Hepatology, Metabolism Research
• Note: For research use only