You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1950029 |
---|---|
Category | Assays and Kits |
Description | Dog β2-MG(Beta-2-Microglobulin) ELISA Kit |
Reactivity | Canine |
Range | 0.78-50 μg/mL |
UniProt ID | P19341 |
Sensitivity | 0.23 μg/mL |
Alternative names | Beta 2M; BMG; B2MG; b2M; Beta-2-Microglobulin Read more... |
Note | For research use only |
Application notes | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog β2-MG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog β2-MG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog β2-MG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog β2-MG in the samples is then determined by comparing the OD of the samples to the standard curve. |
Sample Types | Serum, plasma, tissue homogenates and other biological fluids. |
Expiration Date | Please enquire. |
Filter by Rating